Identification of zygotic and nucellar seedlings, originated by the largest embryo in mango seeds cv Ataulfo, using simple-sequence repeat (SSR)

Mexico is the fourth largest producer of mango and leading exporter worldwide, cv ‘Ataulfo’ area sown in country. New cultivars are required to maintain production, ensure competitiveness, mitigate problems with pests diseases. The purpose present study was identify microsatellite (SSR) markers zygotic nucellar seedlings originated by embryo seeds, cultured vitro. DNA extracted from foliar tissue 18 four different populations, its origin identified six SSRs: MIAC-4, MIAC-5, mMiCIR003, mMiCIR030, LMMA1, LMMA9. number alleles found varied two three, allele size ranged 95 325 bp. Based on polymorphism taking place microsatellites genetic similarity index (GSI) data, more than 15% analyzed were be female parent. Nei’s distance UPGMA cluster analysis, variability among populations 6.2%. Key words: Mangifera indica L., polyembryony, microsatellites.